000 01065 a2200193 4500
020 _a9780763742973
041 _aeng
082 0 _a572.8/7
245 _aDNA sequencing III : dealing with difficult templates
_cEdited by Jan Kieleczawa
260 _bJones and Bartlett Publishers,
_c2009.
_aSudbury, Mass. :
300 _axvi, 184 p.;
_c22 cm.
505 _aControlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins --
650 4 _aDNA
_9132992
650 4 _aNucleotide sequence
_9132993
650 4 _aSequence Analysis -- DNA
_9132994
650 4 _aBase Sequence.
_9132995
650 4 _aTemplates -- Genetic
_9132996
700 1 _aKieleczawa, Jan
_9132997
999 _c272714
_d272650