000 | 01065 a2200193 4500 | ||
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020 | _a9780763742973 | ||
041 | _aeng | ||
082 | 0 | _a572.8/7 | |
245 |
_aDNA sequencing III : dealing with difficult templates _cEdited by Jan Kieleczawa |
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260 |
_bJones and Bartlett Publishers, _c2009. _aSudbury, Mass. : |
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300 |
_axvi, 184 p.; _c22 cm. |
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505 | _aControlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- | ||
650 | 4 |
_aDNA _9132992 |
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650 | 4 |
_aNucleotide sequence _9132993 |
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650 | 4 |
_aSequence Analysis -- DNA _9132994 |
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650 | 4 |
_aBase Sequence. _9132995 |
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650 | 4 |
_aTemplates -- Genetic _9132996 |
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700 | 1 |
_aKieleczawa, Jan _9132997 |
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999 |
_c272714 _d272650 |