TY - BOOK AU - Cavalier,Annie AU - Spehner,Daniele AU - Humbel,Bruno M. TI - Handbook of cryo-preparation methods for electron microscopy T2 - Methods in visualization SN - 9781420006735 (ebook : PDF) PY - 2008/// CY - Boca Raton PB - CRC Press KW - Cryobiology KW - Methodology KW - Electron microscopy KW - Electronic books KW - lcsh N1 - Includes bibliographical references and index; Slam-freezing, metal-mirror freezing / Dani�le Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing leica empact / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai, and Patrick Schultz -- 2-D crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chr�tien -- CEMOVIS, cryo-electron microscopy of vitreous sections / Jacques Dubochet ... [et al.] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof ... [et al.] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. M�sini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Dani�le Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa ... [et al.] -- Immunolabeling of ultra-thin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J. C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte; Also available in print edition; CRC Press (http://www.crcpress.com) UR - http://marc.crcnetbase.com/isbn/9781420006735 ER -