TY - GEN AU - Kieleczawa,Jan TI - DNA sequencing III : dealing with difficult templates SN - 9780763742973 U1 - 572.8/7 PY - 2009/// CY - Sudbury, Mass. PB - Jones and Bartlett Publishers KW - DNA KW - Nucleotide sequence KW - Sequence Analysis -- DNA KW - Base Sequence KW - Templates -- Genetic N1 - Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- ER -