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A Practical Guide to Protein Engineering [electronic resource] / by Tuck Seng Wong, Kang Lan Tee.

By: Wong, Tuck Seng [author.]Contributor(s): Tee, Kang Lan [author.] | SpringerLink (Online service)Material type: Text

TextSeries: Learning Materials in BiosciencesPublisher: Cham : Springer International Publishing : Imprint: Springer, 2020Edition: 1st ed. 2020Description: XV, 202 p. 91 illus., 68 illus. in color. online resourceContent type: text Media type: computer Carrier type: online resourceISBN: 9783030568986Subject(s): Proteins | Biochemical engineering | Biology-Technique | Protein Science | Biochemical Engineering | Biological TechniquesAdditional physical formats: Printed edition:: No title; Printed edition:: No titleDDC classification: 572.6 LOC classification: QD431-431.7Online resources: Click here to access online

Contents:

Literature Search -- Sequence Analysis -- Structural Analysis -- Protein Expression Hosts and Expression Plasmids -- Gene Cloning -- Protein Expression -- Assay -- Gene Mutagenesis -- High-Throughput Screening (HTS) -- Protein Variants Analysis and Characterization -- Continuing from Protein Variants -- Employability.

In: Springer Nature eBookSummary: This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

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Item type	Current library	Call number	Status	Date due	Barcode
Ebooks	Mysore University Main Library		Not for loan

This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

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