Wellcome

DNA sequencing III : dealing with difficult templates Edited by Jan Kieleczawa

Contributor(s): Kieleczawa, JanLanguage: English Publication details: Sudbury, Mass. : Jones and Bartlett Publishers, 2009Description: xvi, 184 p.; 22 cmISBN: 9780763742973Subject(s): DNA | Nucleotide sequence | Sequence Analysis -- DNA | Base Sequence | Templates -- GeneticDDC classification: 572.8/7
Contents:
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins --
Item type: List(s) this item appears in: Sericulture New Arrivals
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Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins --

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